Gene expression patterns in the black blowfly (Phormia regina) as revealed by two-dimensional electrophoresis of proteins. I. Developmental stage-specific and sex-specific differences
- The University of Chicago Pritzker School of Medicine, Il (United States)
- Rutgers University at Camden, NJ (United States)
The black blowfly, Phormia regina, has been implicated in human myiasis and as a contact vector of viral and bacterial diseases present in carrion to which female flies are attracted for egg deposition. Inbred strains of P. regina are an excellent model system for studying gene expression in the developmental stages of such holometabolous dipteran parasites. However, information regarding gene and protein expression patterns in regina is limited. The authors used ISO-DALT high-resolution, two-dimensional electrophoresis with solver staining to establish fundamental protein maps for examination of the stage-specific gene expression patterns in the 615 most abundant proteins of the eggs, first- and third-instar larvae, pupae, and male and female adults. They also used a differential extraction technique to identify the major cuticular proteins of the adults. The results show 48 clearly identifiable stage-specific and sex-specific proteins.
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 7014287
- Journal Information:
- Biochemical Genetics; (United States), Vol. 29:11/12; ISSN 0006-2928
- Country of Publication:
- United States
- Language:
- English
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