Comparison of dna-copying fidelity during repair and semiconservative synthesis by radioactive precursor distribution analysis
The authors compare the fidelity of DNA copying during semiconservative and reparative synthesis under normal conditions and during cortisol-induced activation of free-radical processes, by examining the distribution of radioactivity among DNA pyrimidine isopliths. Radioactivity of nucleotide material in the isopliths was measured by counting in appropriate zones of the chromatograms in toluene scintillator. The investigation shows that injury to DNA of different organs, both directly and as a result of faulty repair, leads to shortening of the pyrimidine isopliths, i.e., to changes in the primary structure of DNA. These data help to explain the simultaneously cytostatic, carcinostatic, and mutagenic action of irradiation, cortisol and hydroxyurea.
- Research Organization:
- N.I. Pirogov Second Moscow Medical Institute
- OSTI ID:
- 6999991
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 100:11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL REPAIR
BIOSYNTHESIS
DNA REPLICATION
RADIORECEPTOR ASSAY
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
HEART
HYDROCORTISONE
HYDROXYUREA
IRRADIATION
LIVER
PRECURSOR
RATS
SCINTILLATION COUNTING
THYMIDINE
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
URIDINE
ADRENAL HORMONES
AMIDES
ANIMALS
AZINES
BIOLOGICAL RECOVERY
BODY
CARDIOVASCULAR SYSTEM
CORTICOSTEROIDS
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
GLANDS
GLUCOCORTICOIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PREGNANES
PYRIMIDINES
RECOVERY
REPAIR
RIBOSIDES
RODENTS
STEROID HORMONES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
URACILS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques