Separation of estrogens and polycyclic aromatic hydrocarbons by high performance thin-layer chromatography

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:6995493

Khatib, S

High performance thin-layer chromatography (HPTLC) combined with scanning densitometry was used for the determination of polycyclic aromatic hydrocarbons (PAHs) in environmental samples and for the separation of clinically important estrogenic hormones. Chromatographic and spectroscopic conditions were optimized for the analysis of the PAHs including the selection of the stationary phase, mobile phase composition, development technique, emission response ratios, and the conditions employed for scanning densitometry. The best results were obtained using octadesiylsilanized silica gel plates, methanol/water/acetonitrile (5:1:1) as the mobile phase, and applying multiple development with the plate cutting technique. Emission response ratios and normalized emission ratios were used for qualitative identification, and normal calibration and two-point calibration methods were applied for quantitative measurement. The PAHs were isolated from environmental samples by Soxhlet or ultrasonic extraction procedures, followed by liquid-liquid distribution between dimethylsulfoxide and hexane, and final sample fractionation by silica gel chromatography. The separation conditions for a mixture of nine mammalian estrogens by HPTLC were also optimized. This mixture was separated using silica gel plates, a mobile phase composed of cyclohexane/ethyl acetate or cyclohexane/tetrahydrofuran (3:1), and by using the multiple development with plate cutting technique.

• Research Organization: Wayne State Univ., Detroit, MI (USA)
• OSTI ID: 6995493
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
BEVERAGES
CHEMICAL ANALYSIS
ESTROGENS
CHROMATOGRAPHY
EXHAUST GASES
PARTICULATES
POLYCYCLIC AROMATIC HYDROCARBONS
SEDIMENTS
WATER
INTERNAL COMBUSTION ENGINES
AROMATICS
ENGINES
FLUIDS
FOOD
GASEOUS WASTES
GASES
HEAT ENGINES
HORMONES
HYDROCARBONS
HYDROGEN COMPOUNDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PARTICLES
SEPARATION PROCESSES
STEROID HORMONES
WASTES
520200* - Environment
Aquatic- Chemicals Monitoring & Transport- (-1989)
500200 - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)
510200 - Environment
Terrestrial- Chemicals Monitoring & Transport- (-1989)
550200 - Biochemistry