Alkoxyresorufin metabolism in white-footed mice at relevant environmental concentrations of Aroclor 1254

Journal Article · Thu Oct 01 00:00:00 EDT 1992 · Fundamental and Applied Toxicology; (United States)

DOI:https://doi.org/10.1016/0272-0590(92)90184-J· OSTI ID:6994806

Simmons, G J; McKee, M J ^[1]

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1. Southern Illinois Univ., Carbondale (United States)

Recent investigations have detected polychlorinated biphenyl (PCB) body burdens in wild white-footed mice (Peromyscus leucopus) captured at hazardous waste sites. Insufficient information is currently available to interpret the toxicological significance of these body burdens. In an effort to provide this information, we investigated hepatic changes and PCB body burdens in white-footed mice following a 21-day dietary exposure to a PCB mixture, Aroclor 1254. Dietary concentrations tested were 0, 2.5, 25, 50, and 100 mg Aroclor 1254/kg diet (reported as ppm). Liver weights were significantly increased at all concentrations except 2.5 ppm. Ethoxyresorufin O-dealkylase (EROD) activity, an aryl hydrocarbon hydroxylase-type substrate, was significantly increased at all PCB concentrations, but the dose-response tended to plateau above 25 ppm. Pentoxyresorufin O-dealkylase (PROD) activity, a putative phenobarbital-type substrate, was significantly increased in a dose-dependent manner at 25 ppm PCB and above, with no plateau response. Pentobarbital sleep time was significantly decreased at 25 ppm, but not at 2.5 ppm. Results indicate white-footed mice undergo a mixed-type induction pattern following exposure to Aroclor 1254, with EROD the most sensitive indicator of PCB exposure. This investigation identified a no observed effect concentration for liver weights and PROD activity at 2.5 ppm in the diet which is equivalent to a body burden of 2.0 mg Aroclor 1254/kg wet wt of mice; the no observed effect concentration for EROD is below these levels. These results support the use of EROD, PROD, and liver weight as biomarkers of PCB exposure in field-captured rodents.

• OSTI ID: 6994806
• Journal Information: Fundamental and Applied Toxicology; (United States), Vol. 19:3; ISSN 0272-0590
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
MICE
BIOLOGICAL MARKERS
BODY BURDEN
POLYCHLORINATED BIPHENYLS
BIOLOGICAL EFFECTS
DOSE-RESPONSE RELATIONSHIPS
TOXICITY
GAS CHROMATOGRAPHY
HAZARDOUS MATERIALS
INVESTIGATIONS
LIPIDS
LIVER
METABOLISM
OXIDOREDUCTASES
SLEEP
ADMINISTRATIVE PROCEDURES
ANIMALS
AROMATICS
BODY
CHLORINATED AROMATIC HYDROCARBONS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
MAMMALS
MATERIALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PROTEINS
RODENTS
SEPARATION PROCESSES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550500 - Metabolism