Xanthine oxidase inhibition attenuates ischemic-reperfusion lung injury

Journal Article · Sun May 01 00:00:00 EDT 1988 · J. Surg. Res.; (United States)

DOI:https://doi.org/10.1016/0022-4804(88)90159-X· OSTI ID:6971780

Lynch, M J; Grum, C M; Gallagher, K P; Bolling, S F; Deeb, G M; Morganroth, M L

Ischemic-reperfusion lung injury is a factor potentially limiting the usefulness of distant organ procurement for heart-lung transplantation. Toxic oxygen metabolites are considered a major etiologic factor in reperfusion injury. Although oxygen-free radicals may be generated by many mechanisms, we investigated the role of xanthine oxidase in this injury process by using lodoxamide, a xanthine oxidase inhibitor, to inhibit ischemic-reperfusion injury in an isolated rat lung model. Isolated rat lungs were perfused with physiologic salt solution (PSS) osmotically stabilized with Ficoll until circulating blood elements were nondetectable in the pulmonary venous effluent. Lungs were rendered ischemic by interrupting ventilation and perfusion for 2 hr at 37/sup 0/C. After the ischemic interval, the lungs were reperfused with whole blood and lung injury was determined by measuring the accumulation of /sup 125/I-bovine serum albumin in lung parenchyma and alveolar lavage fluid as well as by gravimetric measurements. Lung effluent was collected immediately pre- and postischemia for analysis of uric acid by high-pressure liquid chromatography. Lodoxamide (1 mM) caused significant attenuation of postischemic lung injury. Uric acid levels in the lung effluent confirmed inhibition of xanthine oxidase. Protection from injury was not complete, however, implying that additional mechanisms may contribute to ischemic-reperfusion injury in the lung.

• Research Organization: Univ. of Michigan Hospitals, Ann Arbor (USA)
• OSTI ID: 6971780
• Journal Information: J. Surg. Res.; (United States), Vol. 44:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ISCHEMIA
ETIOLOGY
LUNGS
INJURIES
OXIDOREDUCTASES
BIOLOGICAL EFFECTS
ALBUMINS
BIOLOGICAL MODELS
BLOOD FLOW
ENZYME INHIBITORS
INHIBITION
IODINE 125
LIQUID COLUMN CHROMATOGRAPHY
OXYGEN
PERFUSED ORGANS
RADICALS
RATS
TRACER TECHNIQUES
URIC ACID
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR DISEASES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYMES
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PROTEINS
PURINES
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
VASCULAR DISEASES
VERTEBRATES
XANTHINES
550901* - Pathology- Tracer Techniques