Analysis of the synthetic pyrethroids, permethrin and 1(R)-phenothrin, in grain using a monoclonal antibody-based test

Journal Article · Wed Jul 01 00:00:00 EDT 1992 · Journal of Agricultural and Food Chemistry; (United States)

DOI:https://doi.org/10.1021/jf00019a041· OSTI ID:6969124

Skerritt, J H; Hill, A S ^[1]; McAdam, D P ^[2]; Stanker, L H ^[3]

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1. CSIRO Division of Plant Industry, North Ryde (Australia)
2. CSIRO Division of Plant Industry, Canberra (Australia)
3. University of California, Livermore (United States)

A monoclonal antibody generated to the synthetic pyrethroid-related hapten, (3-phenoxybenzyl)-2,2-dimethylcyclopropane-1, 3-dicarboxylate-protein conjugate, was used to develop assays for determinations of permethrin and 1(R)-phenothrin in wheat grain and flour milling fractions. The earlier 3-h assay was simplified using two approaches. The antibody was directly conjugated to the enzyme horseradish peroxidase (HRP), which removes a separate incubation and washing step from the assay. Also, an assay has been developed using microwell-bound monoclonal antibody and a HRP-labeled 3-phenoxybenzoic acid derivative. These assay formats have advantages in increased sensitivity and, in the case of the latter assay, accuracy with grain and flour samples. The most sensitive assay format could detect 1.5 ng/mL permethrin; 50% inhibition of antibody binding occurred at 10 ng/mL. These values corresponded to 75 and 500 ppb, respectively, in the original wheat sample. Methanol was the most effective pyrethroid extractant. Use of a simple cleanup procedure for ground grain extracts improved ELISA accuracy but could by omitted for screening purposes.

• OSTI ID: 6969124
• Journal Information: Journal of Agricultural and Food Chemistry; (United States), Vol. 40:7; ISSN 0021-8561
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GRAIN DISINFESTATION
INSECTICIDES
ENZYME IMMUNOASSAY
AGRICULTURE
CEREALS
MONOCLONAL ANTIBODIES
PEROXIDASES
SOLVENT EXTRACTION
ANTIBODIES
BIOASSAY
DISINFESTATION
ENZYMES
EXTRACTION
GRAMINEAE
IMMUNOASSAY
LILIOPSIDA
MAGNOLIOPHYTA
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PESTICIDES
PLANTS
PROTEINS
SEPARATION PROCESSES
550200* - Biochemistry