Selective changes in expression of HLA class I polymorphic determinants in human solid tumors

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.17.6719· OSTI ID:6968800

Natali, P G; Nicotra, M R; Bigotti, A; Venturo, I; Giacomini, P ^[1]; Marcenaro, L; Russo, C ^[2]

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1. Regina Elena Cancer Institute, Rome (Italy)
2. Cornell Univ. Medical College, New York, NY (USA)

Analysis of surgical biopsies with monoclonal antibodies (mAbs) to framework determinants of major histocompatibility complex class I antigens has shown that malignant transformation is frequently associated with a marked loss of these cell surface molecules. The present study sought to determine whether more selective losses of major histocompatibility complex class I expression occur. Multiple specimens from 13 different types of primary and metastatic tumors were tested utilizing mAb BB7.2, which recognizes a polymorphic HLA-A2 epitope. In each case, expression of HLA-A,B,C molecules was determined by testing with mAb W6/32 directed to a framework HLA class I determinant. The authors have found that in HLA-A2-positive patients, HLA-A2 products are not detectable or are reduced in their expression in 70-80% of endometrial, colorectal, mammary, and renal tumors; in 40-60% of soft-tissue, skin, ovary, urinary bladder, prostate, and stomach tumors; and in 25-30% of melanomas and lung carcinomas tested. All tumors expressed the framework HLA-A,B.C determinant. The HLA-A2 epitope recognized by mAb BB7.2 is located in a portion of the HLA-A2 molecule postulated to react with the T-cell receptor. The selective loss of an HLA class I polymorphic epitope shown in this study may explain the mechanism by which tumor cells escape both T-cell recognition and natural killer cell surveillance.

• OSTI ID: 6968800
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:17; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
TUMOR CELLS
ONCOGENIC TRANSFORMATIONS
ANTIGENS
CYTOCHEMISTRY
EPITHELIUM
FLUORESCENCE
IMMUNOLOGY
INTESTINES
KIDNEYS
LYMPHOCYTES
METHIONINE
MONOCLONAL ANTIBODIES
PROSTATE
RECEPTORS
RECTUM
SULFUR 35
UTERUS
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
EVEN-ODD NUCLEI
FEMALE GENITALS
GASTROINTESTINAL TRACT
GLANDS
ISOTOPES
LARGE INTESTINE
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
MALE GENITALS
MATERIALS
MEMBRANE PROTEINS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
SULFUR ISOTOPES
TISSUES
550201* - Biochemistry- Tracer Techniques