Mutagenicity of six DMSO and Tween 80-solvated mild gasification products in Salmonella typhimurium

Conference · Wed Jan 01 00:00:00 EST 1992

OSTI ID:6943799

Stamm, S C; Zhong, B Z; Bryant, E D; Ong, T ^[1]; Johnson, R; Sharp, S ^[2]

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1. National Inst. for Occupational Safety and Health, Morgantown, WV (United States). Div. of Respiratory Disease Studies
2. USDOE Morgantown Energy Technology Center, WV (United States)

gasification is an emerging coal-conversion process that is being developed by the United States Department of Energy and private industry to meet future energy needs. Products of this process are being submitted to NIOSH in Morgantown, WV, for the evaluation of their genotoxic activity. Six mild gasification products, solvated in both DMSO and Tween 80, have been assayed for mutagenic activity in the preincubation modification of the Ames Salmonella assay system using tester strains TA98 and TA1OO with and without S9 metabolic acbvation. No significant mutagenic activity was detected in these samples when DMSO was used as the solvent vehicle; however, two of the six samples indicated positive mutagenic activity on tester strain TA98 with S9 when solvated in Tween 80. The positive control 2-aminoanthracene, an aromatic amine, demonstrated a significant increase in response when assayed using Tween 80 as a solvent as compared to DMSO. These results suggest that mutagen/solvent interactons may be occurring in the testing of these coal-derived samples and the positive control in the Ames assay. Since past studies have indicated that aromatic amines account for a large percentage of the mutagenic activity of coal-derived materials, the solitary use of DMSO as a solvent for mutagenicity determination must be questioned for these and other chemically related materials.

• Research Organization: National Inst. for Occupational Safety and Health, Morgantown, WV (United States). Div. of Respiratory Disease Studies
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AI21-89MC26018
• OSTI ID: 6943799
• Report Number(s): CONF-9203209-1; ON: DE92018645
• Resource Relation: Conference: 1992 EMS meeting on poster elements, Reno, NV (United States), 15-19 Mar 1992
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
01 COAL, LIGNITE, AND PEAT
COAL LIQUIDS
MUTAGENESIS
DMSO
MUTAGEN SCREENING
RESPONSE MODIFYING FACTORS
POLYCYCLIC AROMATIC AMINES
COAL GASIFICATION
EXPERIMENTAL DATA
SALMONELLA
SURFACTANTS
AMINES
BACTERIA
DATA
FLUIDS
GASIFICATION
INFORMATION
LIQUIDS
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
SCREENING
SULFOXIDES
THERMOCHEMICAL PROCESSES
560300* - Chemicals Metabolism & Toxicology
010404 - Coal
Lignite
& Peat- Gasification
016000 - Coal
Lignite
& Peat- Health & Safety