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Title: Sequence analysis of N-ethyl-N-nitrosourea-induced vermilion mutations in Drosophila melanogaster

The mutational specificity of N-ethyl-N-nitrosourea (ENU) was determined in Drosophila melanogaster using the vermilion locus as a target gene. 25 mutants (16 F{sub 1} and 9 F{sub 2} mutants) were cloned and sequenced. Only base-pair changes were observed; three of the mutants represented double base substitutions. Transition mutations were the most prominent sequence change: 61% were GC {yields} AT and 18% AT {yields} GC substitutions. Both sequence changes can be explained by the miscoding properties of the modified guanine and thymine bases. A strong bias of neighboring bases on the occurrence of the GC {yields} AT transitions or a strand preference of both types of transition mutations was not observed. The spectrum of ENU mutations in D. melanogaster includes a significant fraction (21%) of transversion mutations. The data indicate that like in other prokaryotic and eukaryotic systems also in D. melanogaster the O{sup 6}-ethylguanine adduct is the most prominent premutational lesion after ENU treatment. The strong contribution of the O{sup 6}-ethylguanine adduct to the mutagenicity of ENU possibly explains the absence of distinct differences between the type of mutations observed in the F{sub 1} and F{sub 2} mutants.
Authors:
;  [1] ; ;  [2] ;  [3]
  1. (State Univ. of Leiden (Netherlands) J.A. Cohen Institute (Netherlands))
  2. (State Univ. of Leiden (Netherlands))
  3. (Univ. of North Carolina, Chapel Hill (USA))
Publication Date:
OSTI Identifier:
6936612
Resource Type:
Journal Article
Resource Relation:
Journal Name: Genetics; (USA); Journal Volume: 123:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DROSOPHILA; MUTAGENESIS; GENE MUTATIONS; DNA SEQUENCING; NITROSOUREAS; GENETIC EFFECTS; DNA ADDUCTS; DNA BASE TRANSITIONS; GUANINE; THYMINE; ADDUCTS; AMINES; ANIMALS; AROMATICS; ARTHROPODS; AZAARENES; AZINES; BIOLOGICAL EFFECTS; DIPTERA; FLIES; FRUIT FLIES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INSECTS; INVERTEBRATES; MUTATIONS; NITROSO COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PURINES; PYRIMIDINES; STRUCTURAL CHEMICAL ANALYSIS; URACILS 560300* -- Chemicals Metabolism & Toxicology