Sister chromatid exchange analysis in lung and peripheral blood lymphocytes of mice exposed to methyl isocyanate by inhalation

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860090105· OSTI ID:6934723

Kligerman, A D; Campbell, J A; Erexson, G L; Allen, J W; Shelby, M D

Mice were exposed to 1, 3, or 6 ppm methyl isocyanate (MIC) for 6 hr/day for four consecutive days. Lung cells and peripheral blood lymphocytes (PBLs) were removed and cultured for analysis of sister chromatid exchange (SCE) and cell cycle kinetics. MIC caused a small but significant increase in SCE frequency of cultured lung cells from mice exposed to 1, 3, or 6 ppm MIC. MIC did not significantly increase SCE levels in PBLs of mice exposed to concentrations as high as 6 ppm. In cultured PBLs, MIC had a stimulatory effect on cell cycling rates as measured by the replicative index, and it caused a significant reduction in mononuclear leucocyte counts and the mitotic indices.

• Research Organization: Environmental Health Research and Testing, Inc., Research Triangle Park, NC
• OSTI ID: 6934723
• Journal Information: Environ. Mutagen.; (United States), Vol. 9:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL CYCLE
KINETICS
ISOCYANATES
BIOLOGICAL EFFECTS
INHALATION
CHRONIC EXPOSURE
IN VITRO
LUNGS
LYMPHOCYTES
MICE
MITOTIC INDEX
SISTER CHROMATID EXCHANGES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
INTAKE
LEUKOCYTES
MAMMALS
MATERIALS
MUTATIONS
ORGANS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology