Rapid presumptive identification of the Mycobacterium tuberculosis-bovis complex by radiometric determination of heat stable urease
Simple and rapid Bactec methodologies for the determination of neat (unaltered) and heat stable urease activity of mycobacteria are presented. Clinical isolates (63) and stock cultures (32)--consisting of: M. tuberculosis (19), M. bovis (5), M. kansasii (15), M. marinum (4), M. simiae (3), M. scrofulaceum (16), M. gordonae (6), M. szulgai (6), M. flavescens (1), M. gastri (1), M. intracellulare (6), M. fortuitum-chelonei complex (12), and M. smegmatis (1)--were tested for neat urease activity by Bactec radiometry. Mycobacterial isolates (50-100 mg wet weight) were incubated at 35 degrees C for 30 minutes with microCi14C-urea. Urease-positive mycobacteria gave Bactec growth index (GI) values greater than 100 units, whereas urease-negative species gave values less than 10 GI units. Eighty-three isolates possessing neat urease activity were heated at 80 degrees C for 30 minutes followed by incubation at 35 degrees C for 30 minutes with 1 microCi14C-urea. Mycobacterium tuberculosis-bovis complex demonstrated heat-stable urease activity (GI more than 130 units) and could be distinguished from mycobacteria other than tuberculosis (MOTT), which gave GI values equal to or less than 40 units.
- Research Organization:
- Department of Pathology, Schumpert Medical Center, Shreveport, LA
- OSTI ID:
- 6915555
- Journal Information:
- Am. J. Med. Technol.; (United States), Vol. 49:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MYCOBACTERIUM TUBERCULOSIS
RADIOMETRIC ANALYSIS
UREASE
ENZYME ACTIVITY
CARBON 14 COMPOUNDS
SEPARATION PROCESSES
TRACER TECHNIQUES
AMIDINASES
BACTERIA
CHEMICAL ANALYSIS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
MYCOBACTERIUM
NON-PEPTIDE C-N HYDROLASES
QUANTITATIVE CHEMICAL ANALYSIS
550701* - Microbiology- Tracer Techniques