Stimuli of pepsinogen secretion from frog isolated peptic cells
The authors have previously studied pepsinogen (Pg) secretion from isolated intact esophageal mucosa of the bullfrog R. catesbeiana. By stimulus-response studies using agonists and antagonists they characterized specific stimulation of cholinergic, adrenergic and peptidergic receptors and interaction of cAMP and Ca/sup 2 +/ dependent pathways. To understand cell mechanisms more definitively and to relate these to morphology it was necessary to isolate peptic cells. Esophageal mucosa was digested with 0.1% collagenase for 80-100 min and sieved through teflon mesh. One esophagus yielded approximately 10/sup 7/ cells, 70% pure and 89 +/- 5% viable. Basal secretion was 3% of Pg content/hr. The cells responded to graded concentrations of bombesin, bethanechol, IBMX, 8Br-cAMP, forskolin, TPA (12-0-tetradecanoyl phorbol 13 acetate) and A23187. The response to (TPA + A23187) was double the additive single output values; (TPA + A23187 + forskolin) stimulated secretion of more than double the sum of the 3 component stimuli. In calcium and magnesium-free medium, the A23187 response and the synergistic response of combinations were both lost. They have identified 3 messengers for Pg cell stimulation - cAMP, Ca/sup 2 +/ mobilization and protein kinase C - each of which can be separately stimulated, and when combined are strongly synergistic.
- Research Organization:
- Univ. of Alabama, Birmingham
- OSTI ID:
- 6914084
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PEPSIN
SECRETION
PHORBOL ESTERS
BIOLOGICAL EFFECTS
AMP
CALCIUM COMPOUNDS
ESOPHAGUS
FROGS
MUCOUS MEMBRANES
PHOSPHOTRANSFERASES
SYNERGISM
ACID PROTEINASES
ALKALINE EARTH METAL COMPOUNDS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BODY
CARCINOGENS
DIGESTIVE SYSTEM
ENZYMES
ESTERS
HYDROLASES
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PHOSPHORUS-GROUP TRANSFERASES
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology