Human placental Na/sup +/, K/sup +/-ATPase. cap alpha. subunit: cDNA cloning, tissue expression, DNA polymorphism, and chromosomal localization

Journal Article · Sun Nov 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.22.7901· OSTI ID:6879523

Chehab, F F; Kan, Y W; Law, M L; Hartz, J; Kao, F T; Blostein, R

A 2.2-kilobase clone comprising a major portion of the coding sequence of the Na/sup +/, K/sup +/-ATPase ..cap alpha.. subunit was cloned from human placenta and its sequence was identical to that encoding the ..cap alpha.. subunit of human kidney and HeLa cells. Transfer blot analysis of the mRNA products of the Na/sup +/, K/sup +/-ATPase gene from various human tissues and cell lines revealed only one band (approx. = 4.7 kilobases) under low and high stringency washing conditions. The levels of expression in the tissues were intestine > placenta > liver > pancreas, and in the cell lines the levels were human erythroleukemia > butyrate-induced colon > colon > brain > HeLa cells. mRNA was undetectable in reticulocytes, consistent with the authors failure to detect positive clones in a size-selected ( > 2 kilobases) lambdagt11 reticulocyte cDNA library. DNA analysis revealed by a polymorphic EcoRI band and chromosome localization by flow sorting and in situ hybridization showed that the ..cap alpha.. subunit is on the short is on the short arm (band p11-p13) of chromosome 1.

• Research Organization: Univ. of California, San Francisco (USA)
• OSTI ID: 6879523
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:22
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP-ASE
DNA SEQUENCING
RECOMBINANT DNA
DNA-CLONING
AMINO ACID SEQUENCE
AUTORADIOGRAPHY
CHROMOSOMES
GENETIC MAPPING
HELA CELLS
HYBRIDIZATION
INTESTINES
KIDNEYS
LIVER
MAN
MESSENGER-RNA
PANCREAS
PLACENTA
TUMOR CELLS
ACID ANHYDRASES
ANIMAL CELLS
ANIMALS
BODY
CLONING
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
ENDOCRINE GLANDS
ENZYMES
FETAL MEMBRANES
GASTROINTESTINAL TRACT
GLANDS
HYDROLASES
MAMMALS
MAPPING
MEMBRANES
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
PRIMATES
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques