Depressed glucose utilization in lungs of BB wistar spontaneously diabetic rats
Lungs of BB wistar spontaneously diabetic rats were perfused with (/sup 14/C(U))glucose in modified Krebs Ringer bicarbonate medium for 1.5 hours. Lungs from non-diabetic BB Wistar rats were perfused simultaneously and served as controls. The perfusions were terminated by rapid freezing of the tissue in liquid N/sub 2/ followed by separation of surfactant and residual lung fractions. The rates of glucose incorporation into surfactant DSPC, PG, and PE were decreased 4.7, 2.4 and 2.5-fold, respectively, in lungs of spontaneously diabetic rats when expressed as final product specific activities. The rate of glucose incorporation into residual PC was also reduced by 2.3-fold. Expressed as moles incorporated per gram wet weight of lung, incorporations into surfactant DSPC, PG and residual PC were also reduced by 4.1, 6.3 and 3.8-fold respectively. These data; (1) agree with previous studies of the lungs of streptozotocin and alloxan-diabetic rats; (2) show that the depressed glucose utilization for lipid synthesis observed previously is not due to streptozotocin or alloxan toxicity; (3) suggest that the BB Wistar rat will provide a useful model for the study of the effects of insulin-dependent diabetes on lung metabolism.
- Research Organization:
- St. Louis Univ. School of Medicine, MO
- OSTI ID:
- 6845567
- Report Number(s):
- CONF-8604222-; TRN: 87-008694
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIABETES MELLITUS
PATHOGENESIS
GLUCOSE
METABOLISM
BIOLOGICAL MODELS
CARBON 14 COMPOUNDS
LUNGS
PERFUSED ORGANS
RATS
SURFACTANTS
TRACER TECHNIQUES
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
DISEASES
ENDOCRINE DISEASES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLIC DISEASES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques
550901 - Pathology- Tracer Techniques