Processing of asparagine-linked oligosaccharides in HT-29 cells is a function of their state of enterocytic differentiation: an accumulation of Man/sub 9-8/-GlcNAc/sub 2/-Asn species is indicative of an impaired N-glycan trimming in undifferentiated cells

Journal Article · Thu May 05 00:00:00 EDT 1988 · J. Biol. Chem.; (United States)

OSTI ID:6838274

Ogier-Denis, E; Codogno, P; Chantret, I; Trugnan, G

Studies on the regulation of the enterocytic differentiation of the human colon cancer cell line HT-29, which is differentiated in the absence (Glc/sup -/) but not in the presence of glucose (Glc/sup +/), have recently shown that the post-translational processing of sucrase-isomaltase and particularly its glycosylation vary as a function of cell differentiation. Other studies indicate that in undifferentiated HT-29 Glc/sup +/ cells there is an accumulation of UDP-N-acetylhexosamine, which is involved in the glycosylation process. The purpose of the present work is to investigate whether an overall alteration of protein glycosylation is associated with the inability of HT-29 cells to differentiate. At least three alterations are detected: (i) after a 10-min pulse, the incorporation of D-(2-/sup 3/H)mannose in undifferentiated cells is severely reduced, compared to differentiated cells. (ii) After a 24-h period of labeling with D-(2-/sup 3/H)mannose, undifferentiated cells accumulate more than 60% of the radioactivity in the high mannose glycopeptides, whereas differentiated HT-29 Glc/sup -/ cells accumulate only 38%. (iii) The analysis of the high mannose oligosaccharides transferred en bloc from the lipid precursor shows that Man/sub 9-8/-GlcNAc/sub 2/ species accumulate in undifferentiated cells, whereas no such accumulation can be detected in differentiated cells. This glycosylation pattern is consistent with an impairment of the trimming of high mannose into complex glycans. It is concluded that N-glycan processing is correlated with the state of enterocytic differentiation of HT-29 cells.

• Research Organization: Institut National de la Sante et de la Recherche Medicale (France)
• OSTI ID: 6838274
• Journal Information: J. Biol. Chem.; (United States), Vol. 263:13
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CELL DIFFERENTIATION
OLIGOSACCHARIDES
BIOCHEMISTRY
TRITIUM COMPOUNDS
UPTAKE
TUMOR CELLS
ASPARAGINE
INTESTINES
MANNOSE
ALDEHYDES
AMIDES
AMINO ACIDS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMISTRY
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
HEXOSES
LABELLED COMPOUNDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
SACCHARIDES
550201* - Biochemistry- Tracer Techniques