Metabolic activity is necessary for activation of T suppressor cells by B cells

Journal Article · Sun Apr 15 00:00:00 EDT 1990 · Journal of Immunology; (USA)

OSTI ID:6833772

Elkins, K L; Stashak, P W; Baker, P J ^[1]

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1. National Institutes of Health, Rockville, MD (USA)

Ag-primed B cells must express cell-surface IgM, but not IgD or Ia Ag, and must remain metabolically active, in order to activate suppressor T cells (Ts) specific for type III pneumococcal polysaccharide. Ag-primed B cells that were gamma-irradiated with 1000r, or less, retained the ability to activate Ts; however, Ag-primed B cells exposed to UV light were not able to do so. gamma-Irradiated and UV-treated Ag-primed B cells both expressed comparable levels of cell-surface IgM, and both localized to the spleen after in vivo transfer; neither could proliferate in vitro in response to mitogens. By contrast, gamma-irradiated primed B cells were still able to synthesize proteins, whereas UV-treated primed B cells could not. These findings suggest that in order for Ag-primed B cells to activate Ts, they must (a) express cell-associated IgM (sIgM) antibody bearing the idiotypic determinants of antibody specific for type III pneumococcal polysaccharide, and (b) be able to synthesize protein for either the continued expression of sIgM after cell transfer, or for the elaboration of another protein molecule that is also required for the activation of Ts; this molecule does not appear to be Ia Ag.

• OSTI ID: 6833772
• Journal Information: Journal of Immunology; (USA), Vol. 144:8; ISSN 0022-1767
• Country of Publication: United States
• Language: English

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