Phagocytosis and solubilization of fixed cells by metastatic hamster embryo fibroblasts, Nil2C2

Journal Article · Tue May 01 00:00:00 EDT 1984 · Cancer Res.; (United States)

OSTI ID:6817054

Sakiyama, H; Nishino, Y; Nishimura, K; Noda, Y; Otsu, H

When Nil2C2, a metastatic clone derived from hamster embryo fibroblasts (Nil), was inoculated over (/sup 3/H)leucine-labeled fixed cells, Nil2C2 cells solubilized and phagocytosed fixed cells, and the radioactivity was released into the culture medium as trichloroacetic acid-soluble fragments. The solubilization of fixed cells was dependent on both the time of incubation of living cells with fixed cells and the number of living cells inoculated. Nil2C2 cells were shown by autoradiographic and electron microscopic studies to peel off fixed cells and ingest them as large fragments. The solubilization of fixed cells was significantly decreased when plasminogen was depleted from the culture medium. Protease inhibitors such as leupeptin, epsilon-aminocaproic acid, and soybean trypsin inhibitor partially inhibited the proteolysis and phagocytosis of Nil2C2 cells. Mouse peritoneal macrophages activated by Salmonella typhimurium solubilized fixed cells after the addition of 12-O-tetradecanoylphorbol-13-acetate. However, they did not phagocytose fixed cells as large fragments.

• Research Organization: Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba-shi, Japan
• OSTI ID: 6817054
• Journal Information: Cancer Res.; (United States), Vol. 44:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EMBRYONIC CELLS
PHAGOCYTOSIS
AUTORADIOGRAPHY
CELL CULTURES
ELECTRON MICROSCOPY
FIBROBLASTS
HAMSTERS
LEUCINE
MACROPHAGES
MONOCARBOXYLIC ACIDS
ONCOGENIC TRANSFORMATIONS
PHORBOL ESTERS
SALMONELLA
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BACTERIA
CARBOXYLIC ACIDS
CARCINOGENS
CONNECTIVE TISSUE CELLS
ESTERS
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
MICROSCOPY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
RODENTS
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques