Increased slow transport in axons of regenerating newt limbs after a nerve conditioning lesion made prior to amputation
The first part of this study shows that axonal density is constant in the limb stump of the next proximal to the area of traumatic nerve degeneration caused by limb amputation. The results of the second part of this work reveal that a nerve conditioning lesion made two weeks prior to amputation is associated with accelerated limb regeneration and that this accelerated limb regeneration is accompanied by an earlier arrival of axons. This is the first demonstration of naturally occurring limb regeneration being enhanced. In this study SCb cytoskeletal proteins were identified and measured using SDS-PAGE and liquid scintillation counting. Proteins were measured at 7, 14, 21, and 28 days after {sup 35}S-methionine injection and the normal rate of SCb transport determined to be 0.19 mm/day. A single axotomy does not enhance the rate of SCb transport but does increase the amount of labeled SCb proteins that are transported. When a conditioning lesion is employed prior to limb amputation and SCb proteins are measured at 7, 14, and 21 days after injection, there is a twofold acceleration in the rate of SCb transport and an increase in the amount of SCb proteins transported in conditioned axons.
- Research Organization:
- Case Western Reserve Univ., Cleveland, OH (USA)
- OSTI ID:
- 6810207
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Neurofilament gene expression: a major determinant of axonal caliber
Injury, nerve, and wound epidermis related electrophoretic and fluorographic protein patterns in forelimbs of adult newts
Related Subjects
LIMBS
BIOLOGICAL REGENERATION
NERVE CELLS
BIOLOGICAL FUNCTIONS
PROTEINS
MEMBRANE TRANSPORT
BIOSYNTHESIS
METHIONINE
SALAMANDERS
SCINTILLATION COUNTING
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BODY
BODY AREAS
CARBOXYLIC ACIDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FUNCTIONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
RECOVERY
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550301* - Cytology- Tracer Techniques