Use of DNA probes to study tetracycline resistance determinants in gram-negative bacteria from swine
Specific {sup 32}P-labeled DNA probes were prepared and used to evaluate the distribution of tetracycline resistance determinants carried by gram-negative enteric bacteria isolated from pigs in 3 swine herds with different histories of antibiotic exposure. Plasmid DNA, ranging in size from 2.1 to 186 Kb, was observed in over 84% of 114 isolates studied. Two of 78 tetracycline resistant strains did not harbor plasmids. The DNA probes were isolated from plasmids pSL18, pRT29/Tn10, pBR322 and pSL106, respectively, and they represented class A, B, C and D tetracycline resistance determinants. Hybridization conditions using 0.5X SSPE at 65{degrees}C minimize cross-hybridization between the different class of tetracycline resistance genes. Cross-hybridization between class A and class C determinants could be distinguished by simultaneous comparison of the intensity of their hybridization signals. Plasmids from over 44% of the tetracycline resistant isolates did not hybridize to DNA probes for the determinants tested. Class B determinant occurred more frequently than class A or C. None of the isolates hybridized with the class D probe.
- Research Organization:
- Kentucky Univ., Lexington, KY (USA)
- OSTI ID:
- 6808664
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL ADAPTATION
TETRACYCLINES
BIOLOGICAL EFFECTS
BACTERIAL DISEASES
DNA HYBRIDIZATION
GENE MUTATIONS
PHOSPHORUS 32
PLASMIDS
SWINE
TRACER TECHNIQUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
DOMESTIC ANIMALS
DRUGS
HYBRIDIZATION
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
PHOSPHORUS ISOTOPES
RADIOISOTOPES
VERTEBRATES
553005* - Agriculture & Food Technology- Animal Husbandry- (1987-)
550201 - Biochemistry- Tracer Techniques