RNA polymerase II interacts with the promoter region of the noninduced hsp70 gene in Drosophila melanogaster cells
By using a protein-DNA cross-linking method, we examined the in vivo distribution of RNA polymerase II on the hsp70 heat shock gene in Drosophila melanogaster Schneider line 2 cells. In heat shock-induced cells, a high level of RNA polymerase II was detected on the entire gene, while in noninduced cells, the RNA polymerase II was confined to the 5' end of the hsp70 gene, predominantly between nucleotides -12 and +65 relative to the start of transcription. This association of RNA polymerase II was apparent whether the cross-linking was performed by a 10-min UV irradiation of chilled cells with mercury vapor lamps or by a 40-microsecond irradiation of cells with a high-energy xenon flash lamp. We hypothesize that RNA polymerase II has access to, and a high affinity for, the promoter region of this gene before induction, and this poised RNA polymerase II may be critical in the mechanism of transcription activation.
- Research Organization:
- Cornell Univ., Ithaca, NY (United States)
- OSTI ID:
- 6776952
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
GENETIC RADIATION EFFECTS
RNA POLYMERASES
ENZYME ACTIVITY
CROSS-LINKING
DNA
DROSOPHILA
GENES
TRANSCRIPTION
ULTRAVIOLET RADIATION
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL REACTIONS
DIPTERA
ELECTROMAGNETIC RADIATION
ENZYMES
FLIES
FRUIT FLIES
GENETIC EFFECTS
INSECTS
INVERTEBRATES
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POLYMERIZATION
RADIATION EFFECTS
RADIATIONS
TRANSFERASES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture