Concentration, physical state, and purity of bacterial endotoxin affect its detoxification by ionizing radiation

Journal Article · Sat Nov 01 00:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576821· OSTI ID:6776601

Csako, G; Tsai, C M; Hochstein, H D; Elin, R J

Increasing concentrations of a highly purified bacterial lipopolysaccharide preparation, the U.S. Reference Standard Endotoxin, were exposed to increasing doses of ionizing radiation from a 60Co source. At identical radiation doses both the structural change and Limulus amebocyte lysate (LAL) reactivity were progressively smaller with increasing concentrations of the lipopolysaccharide in an aqueous medium. Under the experimental conditions used, there was a linear relationship between the endotoxin concentration and radiation dose for the structural changes. In contrast to endotoxin in aqueous medium, endotoxin irradiated in its dry state showed no decrease in LAL reactivity and rabbit pyrogenicity. Endotoxin exposed to radiation in water in the presence of albumin showed a much smaller decrease in LAL and pyrogenic activities than expected. The results show that the concentration, physical state, and purity of endotoxin influence its structural and functional alteration by ionizing radiation.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 6776601
• Journal Information: Radiat. Res.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ENDOTOXINS
CHEMICAL RADIATION EFFECTS
COBALT 60
DETOXIFICATION
ESCHERICHIA COLI
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMISTRY
COBALT ISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MATERIALS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOISOTOPES
TOXIC MATERIALS
TOXINS
YEARS LIVING RADIOISOTOPES
560130* - Radiation Effects on Microorganisms