Conditions supporting repair of potentially lethal damage cause a significant reduction of ultraviolet light-induced division delay in synchronized and plateau-phase Ehrlich ascites tumor cells
Repair of potentially lethal damage (PLD) induced by uv light in synchronized and in plateau-phase cultures of Ehrlich ascites tumor cells was studied by measuring cell survival. In particlar the influence of conditions supporting repair of PLD on growth kinetics was investigated. In synchronized G/sub 1/, S, or G/sub 2/ + M cells as well as in plateau-phase cells, uv light induced, almost exclusively, delay in the next S phase. A significant decrease of this delay was observed when the cells were incubated for 24 hr in balanced salt solution. Repair of PLD after uv irradiation was found to occur in plateau-phase cells and in cells in different phases of the cell cycle provided that after irradiation these were kept under conditions inhibiting cell multiplication (incubation in balanced salt solution or in conditioned medium). The repair time constant t/sub 50/ was significantly higher than those found for X irradiation (5-10 hr compared to 2 hr), and repair was not significantly inhibited by either 20 ..mu..g/ml cycloheximide or 2 mM caffeine in 24 hr.
- Research Organization:
- Institut fur Biophysik and Gesellschaft fur Strahlen- und Umweltforschung, Frankfurt, West Germany
- OSTI ID:
- 6709094
- Journal Information:
- Radiat. Res.; (United States), Vol. 91:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIOLOGICAL REPAIR
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
RADIOINDUCTION
MITOTIC DELAY
ASCITES TUMOR CELLS
CAFFEINE
CELL CYCLE
CULTURE MEDIA
CYCLOHEXIMIDE
DNA
EXTREME ULTRAVIOLET RADIATION
SURVIVAL CURVES
ANALEPTICS
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CENTRAL NERVOUS SYSTEM AGENTS
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
HETEROCYCLIC COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PESTICIDES
PURINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
TUMOR CELLS
ULTRAVIOLET RADIATION
XANTHINES
560121* - Radiation Effects on Cells- External Source- (-1987)