Comparison of two immunoradiometric assays for serum thyrotropin

Conference · Wed May 01 00:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:6706450

Scheinin, B; Drew, H; La France, N; Ladenson, P; Wagner, Jr, H N

An ultra-sensitive TSH assay capable of detecting subnormal TSH levels would be useful in confirming suppressed pituitary function as seen in hyperthyroidism. Two sensitive immunoradiometric TSH assays (IRMA's) were studied to determine how well they distinguished thyrotoxic patients from normal subjects. Serono Diagnostics' method employs three monoclonal antibodies specific for different regions of the TSH molecule with a minimum detectable dose (MDD) limit of 0.1 ..mu..IU/ml. Precision studies using a low TSH control in the 1.8 ..mu..IU/ml range gave CV's of 15.0%. Boots-Celltech Diagnostics method is a two site IRMA using two monoclonal antibodies. The MDD limit is 0.05 ..mu..IU/ml with precision CV's of 29.3% at a TSH control range of 0.62 ..mu..IU/ml. In 24 chemically thyrotoxic patients, the mean serum TSH concentration was significantly lower than in the normal control subjects: for Serono, 0.19 ..mu..IU/ml vs. 2.34 ..mu..IU/ml and for Boots Celltech, 0.18 IU/ml vs 2.06 ..mu..IU/ml. The range of TSH was 0 to 0.5 ..mu..IU/ml in thyrotoxic patients using Serono with the exception of one patient having a TSH value of 0.8 ..mu..IU/ml. The normal range was 0.6 to 6.0 ..mu..IU/ml. For Boots Celltech the thyrotoxic range was 0 to 0.2 ..mu..IU/ml with that same thyrotoxic patient giving a TSH value of 0.7 ..mu..IU/ml with a normal range of 0.6 to 5.0 IU/ml. Serum TSH measurements using both procedures are highly sensitive for distinguishing thyrotoxic patients from normal subjects and are useful to confirm suppressed pituitary function.

• Research Organization: The Johns Hopkins Medical Institutions, Baltimore, MD
• OSTI ID: 6706450
• Report Number(s): CONF-850611-; TRN: 87-015124
• Journal Information: J. Nucl. Med.; (United States), Vol. 26:5; Conference: 32. annual meeting of the Society of Nuclear Medicine, Houston, TX, USA, 2 Jun 1985
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
HYPERTHYROIDISM
DIAGNOSIS
MONOCLONAL ANTIBODIES
DIAGNOSTIC USES
UPTAKE
TRH
DETECTION
RADIOIMMUNOASSAY
TSH
CHEMICAL COMPOSITION
COMPARATIVE EVALUATIONS
CONTROL
DOSE LIMITS
IMMUNE SERUMS
LEVELS
PATIENTS
PITUITARY GLAND
SENSITIVITY
ANTIBODIES
BODY
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOLOGY
SAFETY STANDARDS
STANDARDS
TRACER TECHNIQUES
USES
550601* - Medicine- Unsealed Radionuclides in Diagnostics