RIA of thyroglobulin using monoclonal antibodies: Minimal interference by anti-thyroglobulin autoantibodies
Thyroglobulin (Tg) is considered to be secreted from the thyroid gland with the stimulation of TSH and/or thyroid stimulating immunoglobulins. However its use as a prognostic marker for Graves' disease is hampered by anti-Tg autoantibodies in patients' serum. In order to resolve this drawback, the authors have developed monoclonal antibodies to human Tg with very little cross-reactivities with autoantiobodies. Nine monoclonal antibodies were produced by the immunization with Tg prepared from Graves' thyroid and one of them (IgGl), designated as 59A, showed the highest affinity to Tg (3.6 x 10/sup 40/M/sup -1/) and the least cross-reactivity with anti-Tg autoantibodies. The binding of I-125 labeled 59A to beads coated with Tg was not inhibited by the addition of purified IgG obtained from various thyroid diseases except a few Hashimoto's patients with very high titer of anti-Tg antibodies, although the binding of other monoclonal antibodies to Tg was greatly influenced even in the presence of Graves' IgG. The sensitivity of the assay using 59A was enough to detect 20ng Tg/ml and Tg concentrations, in patients with no detectable anti-Tg antibodies, were comparable to those determined by the conventional RIA kit (Eiken), using radioiodinated Tg and polyclonal rabbit anti-Tg antiserum. Further, the shelf-life of I-125 labeled monoclonal antibody was much longer than the radioiodinated Tg. These results indicated that RIA of Tg using monoclonal antibodies would be useful for measuring Tg values not only in patients with thyroid cancer but also in Graves' disease with anti-Tg autoantibodies.
- Research Organization:
- Kyoto Univ. Hospital, Kyoto
- OSTI ID:
- 6706437
- Report Number(s):
- CONF-850611-; TRN: 87-015125
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 26:5; Conference: 32. annual meeting of the Society of Nuclear Medicine, Houston, TX, USA, 2 Jun 1985
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IODINE 125
DIAGNOSTIC USES
MONOCLONAL ANTIBODIES
CHEMICAL PREPARATION
NEOPLASMS
DIAGNOSIS
THYROGLOBULIN
RADIOIMMUNOASSAY
THYROID
BINDING ENERGY
COATINGS
COMPARATIVE EVALUATIONS
ENDOCRINE DISEASES
IMMUNE SERUMS
IODINATION
LABELLED COMPOUNDS
LIFETIME
RABBITS
RADIOACTIVATION
SENSITIVITY
STIMULATION
STORAGE LIFE
THYROID HORMONES
TSH
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENERGY
GLANDS
GLOBULINS
HALOGENATION
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SYNTHESIS
TRACER TECHNIQUES
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics