Nuclear magnetic resonance studies of biological systems: Applications to liver preservation and metabolism in cultured pituitary tumor cells
This study centers on applications of both {sup 31}P and {sup 13}C nuclear magnetic resonance spectroscopy to two different biological systems. The first application utilizes {sup 31}P NMR to study mobile phospholipids in the MMQ cell line, a pituitary tumor cell line. These measurements characterize membrane phospholipids thought to be part of a RNA-proteolipid complex unique to cellular transformation. The second application utilizes both {sup 31}P and {sup 13}C spectroscopy to study liver preservation and transplantation an a rat model. In this work, several questions were addressed: (1) to what extent do successful preservation solutions slow ATP breakdown (2) can clinically successful preservation conditions ameliorate total nucleotide breakdown (3) to what extent is energy reconstitution following cold storage correlated with transport success and (4) can any spectroscopic parameter be used as a diagnostic indicator of tissue viability
- Research Organization:
- Virginia Univ., Charlottesville, VA (USA)
- OSTI ID:
- 6698502
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ATP
METABOLISM
LIVER
TRANSPLANTS
PHOSPHOLIPIDS
MOLECULAR STRUCTURE
BIOLOGICAL MODELS
CARBON 13
CELL CULTURES
MEMBRANE PROTEINS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PHOSPHORUS 31
PITUITARY GLAND
RATS
TRACER TECHNIQUES
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BODY
CARBON ISOTOPES
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
ESTERS
EVEN-ODD NUCLEI
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MAMMALS
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
RESONANCE
RODENTS
SPECTRA
STABLE ISOTOPES
VERTEBRATES
550501* - Metabolism- Tracer Techniques