(/sup 35/S)autoradiographic study of sulfated GAG accumulation and turnover in embryonic mouse tooth germs
The accumulation of sulfated glycosaminoglycans(GAG) in embryonic mouse molars before, during, and after terminal differentiation of odontoblasts was localized by (/sup 35/S)autoradiography combined with the use of chondroitin ABC lyase. Much more sulfated GAG were accumulated in the dental papilla than in the dental epithelium. High incorporation of (/sup 35/S)sulfate occurred at the epithelio-mesenchymal junction, which is the site of dental basement membrane and predentin. Before terminal differentiation of odontoblasts, the distribution of sulfated GAG was uniform at the basement membrane. After the onset of terminal differentiation of odontoblasts, much more sulfated GAG accumulated at the tip of principal cusps than at the apical (inferior) parts of cusps, and sulfated GAG were then found to be degraded more rapidly at the epithelio-mesenchymal junction than at other parts of the tooth germ. Thus regional variation in the rate of degradation of GAG exists in the tooth germs. Trypsin-isolated dental epithelia cultured in vitro synthesized a new basement membrane that could be labeled with (/sup 3/H)glucosamine but not with /sup 35/SO4(-2). The epithelial-derived basal lamina contains little or no sulfatated GAG.
- OSTI ID:
- 6668566
- Journal Information:
- J. Craniofacial Genet. Dev. Biol.; (United States), Vol. 3:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SACCHARIDES
TISSUE DISTRIBUTION
TEETH
AUTORADIOGRAPHY
ONTOGENESIS
AMINES
BACTERIA
EMBRYONIC CELLS
EMBRYOS
FETAL MEMBRANES
METABOLISM
MICE
SULFUR 35
SULFUR COMPOUNDS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
MICROORGANISMS
NUCLEI
ORAL CAVITY
ORGANIC COMPOUNDS
RADIOISOTOPES
RODENTS
SULFUR ISOTOPES
VERTEBRATES
550801* - Morphology- Tracer Techniques