Origin of increased deoxycytidine excretion into urine of rats bearing Yoshida ascites sarcoma

Journal Article · Fri Jun 01 00:00:00 EDT 1984 · Cancer Res.; (United States)

OSTI ID:6630955

Shimizu, M; Fujimura, S

The metabolism of deoxycytidine (dCyd) and dCyd nucleotides in Yoshida ascites sarcoma (YS) cells and the host rat liver was investigated with reference to the increased excretion of urinary dCyd. Incorporation of (/sup 14/C)orotic acid into the livers of rats at the fifth day after the transplantation of YS cells, was 2 times higher than that into the normal rat livers. After the injection of (/sup 14/C)orotic acid, the ratio of the specific radioactivity of cytidylate to uridylate moieties of the host liver RNA was measured and found to be higher than that of normal rat liver RNA and to be similar to that of YS cell RNA. When (/sup 14/C)orotic acid was injected into rats followed by the transplantation of YS cells, the radioactivities present in the livers disappeared more rapidly than those in the control rat livers. The activities of pyrimidine de novo synthesis enzymes, such as cytidine triphosphate synthetase and cytidine diphosphate reductase, in YS were higher than those in both rat ascites hepatoma AH 7974 and Walker 256 carcinosarcoma, the transplantations of which did not induce increased excretion of dCyd into urine of the hosts. The activities of dCyd kinase and dCyd deaminase in YS cells were lower than those in the other two tumors investigated. The activities of cytidine triphosphate synthetase and cytidine diphosphate reductase in the livers of YS-bearing rats were elevated compared with those in the livers of rat ascites hepatoma AH 7974- or Walker 256 carcinosarcoma-bearing rats and normal rats, while the activities of dCyd kinase, 5'-nucleotidase, and dCyd deaminase were similar between normal rat livers and tumor-bearing rat livers.

• Research Organization: Chiba Cancer Center Research Inst., Japan
• OSTI ID: 6630955
• Journal Information: Cancer Res.; (United States), Vol. 44:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASCITES TUMOR CELLS
METABOLISM
DEOXYCYTIDINE
EXCRETION
NUCLEOTIDES
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
ENZYME ACTIVITY
EXPERIMENTAL NEOPLASMS
HEPATOMAS
LIVER
RATS
RNA
SARCOMAS
TRACER TECHNIQUES
TRANSPLANTS
ANIMAL CELLS
ANIMALS
AZINES
BODY
CLEARANCE
DIGESTIVE SYSTEM
DISEASES
GLANDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
NEOPLASMS
NUCLEIC ACIDS
NUCLEOSIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
TUMOR CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques