A comparison of results obtained by two different chromium-51 methods of determining platelet survival and recovery

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Transfusion (Philadelphia); (USA)

DOI:https://doi.org/10.1046/j.1537-2995.1990.30590296371.x· OSTI ID:6610464

Rock, G; Tittley, P ^[1]

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1. Univ. of Ottawa, Ontario (Canada)

A number of different methods exist that use chromium-51 to determine platelet survival and recovery. A 1986 article in TRANSFUSION described the recommended methods for conducting such studies in the hope of standardizing methodology and permitting interlaboratory comparison of results. The results obtained with the recommended method have been compared with those of our previous method. With the new method, recovery of fresh random-donor platelets prepared in polyolefin bags was 76 +/- 6 percent with a survival of 9.3 +/- 2.8 days (n = 4). After 5 days of storage, these values were 67 +/- 8 percent and 7.8 +/- 1.9 days. Similar values were obtained for fresh and stored platelets collected on automated cell separators. However, when our previous (pre-1986) method of evaluation was used, recovery of fresh platelets in the polyolefin bags was 56 +/- 11 percent, and survival was 8.6 +/- 1.3 days. The 5-day values were 36 +/- 5 percent and 6.9 +/- 2.7 days. These values are significantly lower (p less than 0.05) than those obtained with the newer method, but they are in agreement with those previously reported for other techniques. Thus, the new method consistently gives higher values, possibly as a result of a less harmful labeling procedure.

• OSTI ID: 6610464
• Journal Information: Transfusion (Philadelphia); (USA), Vol. 30:5; ISSN 0041-1132
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
SURVIVAL TIME
BIOLOGICAL HALF-LIFE
BIOLOGICAL RECOVERY
CHROMIUM 51
INTERLABORATORY COMPARISONS
MAN
REGRESSION ANALYSIS
TRACER TECHNIQUES
TRANSFUSIONS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MATHEMATICS
NUCLEI
PRIMATES
RADIOISOTOPES
RECOVERY
STATISTICS
THERAPY
VERTEBRATES
550301* - Cytology- Tracer Techniques