Absorption and metabolism of ( sup 3 H)arachidonic and ( sup 14 C)linoleic acid in essential fatty acid-deficient rats

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · American Journal of Physiology; (USA)

OSTI ID:6610326

Hjelte, L; Melin, T; Nilsson, A; Strandvik, B ^[1]

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1. Huddinge Univ. Hospital, Stockholm (Sweden)

({sup 3}H)arachidonic acid (20:4) and ({sup 14}C)linoleic acid (18:2) were fed in a triolein emulsion to essential fatty acid-deficient (EFAD) rats and to age-matched controls. Tissues were analyzed for radioactivity of different lipid classes after 1, 2, and 4 h. As in earlier studies, control rats retained more ({sup 3}H)20:4 than ({sup 14}C)18:2 in all organs except adipose tissue. In EFAD rats, recovery of ({sup 14}C)18:2 was increased in small intestine, liver, heart, and kidneys. In comparison to controls, EFAD rats retained much more ({sup 14}C)18:2 in phospholipids of these organs. The increase in the incorporation of both {sup 3}H and {sup 14}C into phosphatidylethanolamine was particularly pronounced. Another striking feature was the drastic increase in the retention after 4 h of {sup 14}C in cardiolipin, which is specifically located in the inner mitochondrial membrane. In contrast, incorporation of both {sup 3}H and {sup 14}C into phosphatidylinositol was decreased or unchanged in EFAD rats. Although fecal fat excretion was increased there was no evidence for a malabsorption or an increased retention in intestinal triacyglycerol of the radioactive fatty acids in EFAD rats. The proportion of ({sup 14}C)18:2 that had been converted to ({sup 14}C)20:4 was generally low but increased significantly with time in the liver and intestine of EFAD rats.

• OSTI ID: 6610326
• Journal Information: American Journal of Physiology; (USA), Vol. 259; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
METABOLISM
LINOLEIC ACID
PHOSPHOLIPIDS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL MEMBRANES
INTESTINAL ABSORPTION
INTESTINES
ISOTOPE DILUTION
MITOCHONDRIA
PREGNANCY
QUANTITY RATIO
RATS
SPECIFICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ABSORPTION
ANIMALS
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ESTERS
GASTROINTESTINAL TRACT
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MEMBRANES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
RODENTS
SYNTHESIS
UPTAKE
VERTEBRATES
550501* - Metabolism- Tracer Techniques