Noninvolvement of the X chromosome in radiation-induced chromosome translocations in the human lymphoblastoid cell line TK6

Journal Article · Tue Mar 01 00:00:00 EST 1994 · Radiation Research; (United States)

DOI:https://doi.org/10.2307/3578701· OSTI ID:6593265

Jordan, R; Schwartz, J L ^[1]

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1. Argonne National Lab., IL (United States)

Fluorescence in situ hybridization procedures were used to examine the influence of chromosome locus on the frequency and type of chromosome aberrations induced by [sup 60]Co [gamma] rays in the human lymphoblastoid cell line TK6. Aberrations involving the X chromosome were compared to those involving the similarly sized autosome chromosome 7. When corrected for DNA content, acentric fragments were induced with equal frequency in the X and 7 chromosomes. Dose-dependent increases in chromosomal interchanges involving chromosome 7 were noted, and the frequencies of balanced translocations and dicentrics produced were approximately equal. Chromosome interchanges involving the X chromosome were rare and showed no apparent dose dependence. Thus, while chromosomes 7 and X are equally sensitive to the induction of chromosome breaks, the X chromosome is much less likely to interact with autosomes than chromosome 7. The noninvolvement of the X chromosome in translocations with autosomes may reflect a more peripheral and separate location for the X chromosome in the mammalian nucleus. 20 refs., 2 figs., 1 tab.

• DOE Contract Number: W-31109-ENG-38; FG02-88ER60661
• OSTI ID: 6593265
• Journal Information: Radiation Research; (United States), Vol. 137:3; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
HUMAN CHROMOSOME 7
COMPARATIVE EVALUATIONS
HUMAN X CHROMOSOME
COBALT 60
DICENTRIC CHROMOSOMES
DNA HYBRIDIZATION
DOSE-RESPONSE RELATIONSHIPS
FLUORESCENCE SPECTROSCOPY
GAMMA RADIATION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOTECHNOLOGY
CHROMOSOMES
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
EVALUATION
GENETIC ENGINEERING
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
MUTATIONS
NUCLEI
NUCLEIC ACID HYBRIDIZATION
ODD-ODD NUCLEI
RADIATIONS
RADIOISOTOPES
SPECTROSCOPY
X CHROMOSOME
YEARS LIVING RADIOISOT
560151* - Radiation Effects on Animals- Man
550200 - Biochemistry
550300 - Cytology