Caged and wild fish: Induction of hepatic cytochrome P-450 (CYP1A1) as an environmental biomonitor
- Medical Coll. of Wisconsin, Milwaukee (United States)
- Rutgers, The State Univ. of New Jersey, Piscataway (United States)
- Univ. of Wisconsin, Milwaukee (United States)
Hepatic monooxygenase activity can be induced by many different environmental chemical contaminants, and measurement of this activity has been proposed as an environmental biomonitor. Using in situ caged catfish and largemouth bass, and collected wild killifish, environmental induction of hepatic CYP1A1 was investigated using catalytic enzyme assays, regiospecific metabolism, immunodetection, and nucleic acid hybridization. The purpose of these studies was to evaluate these techniques for detection of CYP1A1 induction as a potential environmental biomonitor of environmental chemical contamination. Exposure of catfish in cages to polyaromatic hydrocarbon- (PAH-) and polychlorinated biphenyl- (PCB-) contaminated river water for two, four, or six weeks resulted in fourfold increases in ethoxyresorufin-O-deethylase (EROD) activity and three- and fivefold increases in immunoreactive CYP1A1 protein and hybridizable CYP1A1 mRNA, respectively, when compared to laboratory water control values. Hybridizable CYP1A1 mRNA in caged largemouth bass increased 5.1-fold at 1 d of exposure. Caged largemouth bass had 5-, 1.4-, and 0.8-fold increases at 3 d and 6-, 2.4-, 0.4-fold increases at 7 d of river water exposure in EROD, immunoreactive CYP1A1 protein, and CYP1A1 mRNA, respectively, when compared to laboratory water control values. Liver of killifish from a 2,3,7,8-tetrachlorodibenzo-p-dioxin-contaminated area had threefold higher EROD activity and similarly elevated immunoreactive CYP1A1 protein, a two- to fourfold increase in CYP1A1 mRNA, and a four- to eightfold increase in 6[beta]-hydroxyprogesterone activity, when compared to killifish livers sampled from a clean site.
- OSTI ID:
- 6589510
- Journal Information:
- Environmental Toxicology and Chemistry; (United States), Vol. 12:5; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHLORINATED AROMATIC HYDROCARBONS
METABOLISM
FISHES
SENSITIVITY
POLYCYCLIC AROMATIC HYDROCARBONS
WATER POLLUTION
BIOLOGICAL INDICATORS
CYTOCHROMES
ENZYME INDUCTION
LIVER
OXYGENASES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BODY
DIGESTIVE SYSTEM
ENZYMES
GENE REGULATION
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PIGMENTS
POLLUTION
PROTEINS
VERTEBRATES
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology