Possible use of porphycenes as a membrane marker for flow cytometric detection of micronuclei

Conference · Fri Jan 01 00:00:00 EST 1993 · Cytometry (Baltimore); (United States)

OSTI ID:6573109

Wessels, J M; Nuesse, M ^[1]

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1. GSF-Institut fuer Biophysikalische Strahlenforschung, Neuherberg (Germany)

For flow cytometric analysis of micronuclei induced by ionizing radiation or chemicals, debris plays an important role for the unambiguous detection of micronuclei. Several flow cytometric parameters have been used for a discrimination of micronuclei and debris (Schreiber et al., Cytometry 13:90-102, 1992). The lipophilic character of porphycenes can be used to selectively stain cellular and nuclear membranes. This chemical property of porphycenes requires the use of liposomes as a carrier. Porphycenes show high extinction coefficients at 360 nm and high fluorescence quantum yields between 600 nm and 750 nm. Due to their excellent photophysical properties they can therefore be used as a fluoroscent dye in combination with DNA specific dyes. Porphycenes (i.e. hydroxyethyl-tris(methoxyethyl)porphycene, HEPn) were used to either stain selectively the debris produced by membrane particles or to stain the membranes of nuclei and micronuclei for a better discrimination of debris and micronuclei.

• OSTI ID: 6573109
• Report Number(s): CONF-9303114-; CODEN: CYTODQ
• Journal Information: Cytometry (Baltimore); (United States), Vol. 6; Conference: 16. congress of the International Society for Analytical Cytology, Colorado Springs, CO (United States), 21-26 Mar 1993; ISSN 0196-4763
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL NUCLEI
BIOLOGICAL RADIATION EFFECTS
DETECTION
MALFORMATIONS
PORPHYRINS
FLUORESCENCE
CYTOLOGICAL TECHNIQUES
DYES
IONIZING RADIATIONS
MEMBRANES
SORTING
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LUMINESCENCE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
RADIATION EFFECTS
RADIATIONS
550300* - Cytology