Influence of chemical effectors of reactive oxygen species and GSH depletion on cell survival following photodynamic treatment and ionizing radiation

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:6545506

Miller, A C

The influence of cellular glutathione (GSH) levels on the response to photodynamic treatment (PDT) in vitro was determined in cells which were depleted of GSH by buthionine sulfoximine (BSO), or which were genetically GSH deficient. The effects of GSH depletion on cellular radiosensitivity were studied in parallel for comparison. BSO treatment which reduced GSH levels in four cell lines (CHO, V-79, EMT6, RIF) to approximately 80% and 30% of controls, or to undetectable levels, uniformly decreased cell survival. This decrease was directly related to GSH depletion. GSH level-dependent aerobic radiosensitization following BSO treatment was found in all four cell lines, and cell survival changes were expressed in the survival curves. Cell survival of GSH deficient human fibroblasts (GM 3877) was decreased following PDT and gamma irradiation when compared to their normal counterparts (GM 5659). Neither BSO nor GSH interfered with cellular porphyrin uptake or singlet oxygen production during the photodynamic process.

• Research Organization: State Univ. of New York, Buffalo (USA)
• OSTI ID: 6545506
• Resource Relation: Other Information: Thesis (Ph. D)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
RADIOSENSITIVITY
CHO CELLS
FIBROBLASTS
GLUTATHIONE
RADIOSENSITIVITY EFFECTS
GAMMA RADIATION
HAMSTERS
LUNGS
MAN
SURVIVAL CURVES
ANIMALS
BODY
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture