Selective binding of human cumulus cell-secreted glycoproteins to human spermatozoa during capacitation in vitro

Journal Article · Fri Jun 01 00:00:00 EDT 1984 · Fert. Steril.; (United States)

OSTI ID:6540327

Tesarik, J; Kopecny, V; Dvorak, M

The results of this study demonstrate that glycoproteins manufactured by human cumulus cells can be detected bound to human spermatozoa incubated in capacitational medium containing the labeled cumulus-cell secretions. Cumulus-cell-secreted glycoproteins were labeled with a mixture of /sup 3/H-methionine and /sup 3/H-tryptophan or with 3H-fucose, and the binding of the labeled compounds to spermatozoa was evaluated by autoradiography. The binding was highly selective, involving only approximately 1% of the samples of spermatozoa used. The results suggest that the binding of cumulus-cell-secreted glycoproteins to spermatozoa may represent a final and highly selective step in human sperm capacitation.

• Research Organization: J.E. Purkyne Univ., Brno, Czechoslovakia
• OSTI ID: 6540327
• Journal Information: Fert. Steril.; (United States), Vol. 41:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
BIOCHEMICAL REACTION KINETICS
LABELLING
SPERMATOZOA
AUTORADIOGRAPHY
GALACTOSE
IN VITRO
METHIONINE
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPTOPHAN
ALDEHYDES
AMINO ACIDS
AROMATICS
AZAARENES
AZOLES
CARBOHYDRATES
CARBOXYLIC ACIDS
DRUGS
GAMETES
GERM CELLS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
INDOLES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
PYRROLES
REACTION KINETICS
SACCHARIDES
550201* - Biochemistry- Tracer Techniques