High-performance liquid chromatography and immunoassay techniques for monitoring urinary metabolites of polycyclic aromatic hydrocarbons
The purpose of this research was to investigate the feasibility of using high performance liquid chromatography (HPLC) and enzyme linked immunosorbent assays (ELISA) as sensitive techniques for monitoring polycyclic aromatic hydrocarbon (PAH) metabolites in human urine. The method was tested using synthesized PAH conjugates as positive markers. Results showed that a PAH conjugate, S-(9,10-dihydro-9-hydroxy-10-phenanthryl)N-acetyl cysteine (PHONAC), present in HPLC effluent could be detected by ELISA at picomole levels, well below the sensitivity of the HPLC UV detector. Analyses of urine from mice dosed with phenanthrene demonstrated that a substance detected by HPLC which was not detected in ELISA tests was the principal phenanthrene metabolite. This substance was not hydrolysed by Beta-glucuronidase. PHONAC was detected by ELISA in mouse urine extracts subjected to HPLC.
- Research Organization:
- American Univ., Washington, DC (USA)
- OSTI ID:
- 6520407
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
POLYCYCLIC AROMATIC HYDROCARBONS
METABOLISM
ENZYME IMMUNOASSAY
GLUCURONIDASE
LIQUID COLUMN CHROMATOGRAPHY
MEASURING METHODS
METABOLITES
MICE
URINE
ANIMALS
AROMATICS
BIOASSAY
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMATOGRAPHY
ENZYMES
GLYCOSYL HYDROLASES
HYDROCARBONS
HYDROLASES
IMMUNOASSAY
MAMMALS
MATERIALS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
RODENTS
SEPARATION PROCESSES
VERTEBRATES
WASTES
560300* - Chemicals Metabolism & Toxicology