3[prime] end maturation of the Chlamydomonas reinhardtii chloroplast atpB mRNA is a two-step process
- Cornell Univ., Ithaca, NY (United States)
The research studied the 3[prime] end maturation of green algae chloroplast atpB mRNA. Most data on transcription termination and 3[prime] end maturation in chloroplasts have been based on in vitro experiments. Newly developed chloroplast transformation techniques have allowed the use of a green algae, Chlamydomonas reinhardtii, to examine chloroplast mRNA 3[prime] end stability determinants and mRNA processing both in vitro and in vivo. The results of this research showed that Chlamydomonas chloroplast protein extracts contain an endonuclease activity that cleaves a synthetic precursor of atpB mRNA 10 nucleotides downstream on the mature 3[prime] end in vitro. Rapid cleavage by this endonuclease is followed by exonucleolytic removal of 10 nucleotides to yield the mature 3[prime] end.
- OSTI ID:
- 6483779
- Journal Information:
- Molecular and Cellular Biology; (United States), Vol. 13:4; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CHLOROPLASTS
MATURATION
RNA
CHLAMYDOMONAS
ENDONUCLEASES
GENES
PHOTOSYNTHETIC REACTION CENTERS
RNA PROCESSING
ALGAE
CELL CONSTITUENTS
CHLOROPHYCOTA
DNA-ASE
ENZYMES
ESTERASES
HYDROLASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLANTS
PROTEINS
UNICELLULAR ALGAE
140505* - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)
550400 - Genetics