Molecular cloning of copper resistance genes from Pseudomonas syringae pv. tomato

Journal Article · Sun Feb 01 00:00:00 EST 1987 · J. Bacteriol.; (United States)

OSTI ID:6470091

Bender, C L; Cooksey, D A

A cosmid library of copper-resistant (Cu/sup r/) Psuedomonas syringe pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases), conferred copper resistance. The PT23 Cu/sup r/ gene(s) was located on pCOP1 by subcloning PstI restriction endonuclease fragments of pCOP1 in the broad-host-range vector pRK404. A subclone containing a 4.4-kilobase PstI fragment conferred Cu/sup r/ on PS61. The Cu/sup r/ gene(s0 was further located by insertional inactivation with Tn5. A subcloned fragment internal to the Cu/sup r/ determinant on pCOP2 was probed to plasmid and chromosomal DNA of four copper-resistant and three copper-sensitive strains of P. syringae pv. tomato. The probe hybridized to plasmids in resistant strains, but showed no detectable homology to copper-sensitive strains.

• Research Organization: Univ. of California, Riverside
• OSTI ID: 6470091
• Journal Information: J. Bacteriol.; (United States), Vol. 169:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PSEUDOMONAS
DNA-CLONING
GENETIC ENGINEERING
COPPER
ENDONUCLEASES
HYBRIDIZATION
PLASMIDS
SENSITIVITY
TOXICITY
BACTERIA
CELL CONSTITUENTS
CLONING
DNA-ASE
ELEMENTS
ENZYMES
ESTERASES
HYDROLASES
METALS
MICROORGANISMS
PHOSPHODIESTERASES
TRANSITION ELEMENTS
550400* - Genetics
560300 - Chemicals Metabolism & Toxicology