Ethanol's effects on neurotransmitter release and intracellular free calcium in PC12 cells
The effect of ethanol on muscarine-stimulated release of (/sup 3/H)NE was studied using the rat pheochromocytoma cell line, PC12. At concentrations of 25 mM and above, ethanol produced a dose dependent inhibition of muscarine-stimulated release of (/sup 3/H)NE. The inhibition of muscarine-stimulated transmitter release occurred in the absence of any effect of ethanol on (/sup 3/H)NE uptake, metabolism or on muscarinic binding to the cells. However, ethanol produced an inhibition of muscarine-stimulated elevation of intracellular free Ca2+ which corresponded with the inhibition of transmitter release. At concentrations greater than 100 mM, ethanol produced both a stimulation of the release of (/sup 3/H)NE as well as an increase in intracellular free Ca2+. The increase in basal transmitter release and intracellular free Ca2+ occurred independent of the inhibition by ethanol of muscarine-stimulated elevation of intracellular free Ca2+ or transmitter section. These results demonstrate the relationship of the effects of ethanol on cellular free Ca2+ and neurotransmitter release.
- Research Organization:
- National Institute on Alcohol Abuse and Alcoholism, Rockville, MD (USA)
- OSTI ID:
- 6400661
- Journal Information:
- Adv. Alcohol Subst. Abuse; (United States), Vol. 7:3-4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Potentiation of lead-induced cell death in PC12 cells by glutamate: Protection by N-acetylcysteine amide (NACA), a novel thiol antioxidant
Effect of chloroquine on biosynthesis, processing and secretion of proteins from PC12 cells
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CALCIUM
SECRETION
ETHANOL
BIOLOGICAL EFFECTS
NORADRENALINE
BIOELECTRICITY
INHIBITION
METABOLISM
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ADRENAL HORMONES
ALCOHOLS
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARDIOTONICS
CARDIOVASCULAR AGENTS
DRUGS
ELECTRICITY
ELEMENTS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METALS
NEUROREGULATORS
ORGANIC COMPOUNDS
RODENTS
SYMPATHOMIMETICS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques