Characterization of cDNAs of the human pregnancy-specific beta1-glycoprotein family, a new subfamily of the immunoglobulin gene superfamily
- Georgetown Univ. Medical Center, Washington, DC (USA)
Three highly homologous cDNAs encoding human pregnancy-specific {beta}1-glycoprotein (SP1) were isolated from a human placental cDNA library. These cDNAs share >90% nucleotide homology in their coding sequences, and >79% of the encoded amino acids are homologous. Proteins encoded by these cDNAs are very similar to members of the carcinoembryonic antigen family and contain repeating domains, conserved disulfided bridges, and {beta}-sheet structure typical of the immunoglobulin gene superfamily. However, the high degree of sequence homology and relatively lesser degree of glycosylation among the SP1 proteins suggest that they exist as a unique family instead of being members of the CEA family. Both soluble and potentially membrane-bound forms of SP1 proteins were present in the placenta. Northern blot analysis using specific probes confirmed the expression of multiple mRNA species in human term placenta.
- OSTI ID:
- 6325450
- Journal Information:
- Biochemistry; (USA), Vol. 29:11; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLYCOPROTEINS
AMINO ACID SEQUENCE
MESSENGER-RNA
DNA HYBRIDIZATION
RECOMBINANT DNA
DNA SEQUENCING
BLOOD SERUM
CARCINOEMBRYONIC ANTIGEN
IMMUNOGLOBULINS
IODINE 125
PLACENTA
PREGNANCY
WOMEN
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
ELECTRON CAPTURE RADIOISOTOPES
FEMALES
FETAL MEMBRANES
GLOBULINS
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MEMBRANES
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques