Selenite-induced variation in glutathione peroxidase activity of three mammalian cell lines: no effect on radiation-induced cell killing or DNA strand breakage
The selenium-dependent glutathione peroxidase activities of three mammalian cell lines, HT29, P31, and N-18, cultured in medium with low serum content, increased about 2-, 5-, and 40-fold, respectively, after supplementation with 100 nM selenite. Catalase, CuZn superoxide dismutase, and Mn superoxide dismutase activities were not generally influenced by selenite supplementation, and there was only a minor nonselenium-dependent glutathione peroxidase activity in the investigated cell lines. Gamma-irradiated control and selenite-supplemented cells showed no changes in the surviving fractions, as estimated by clonogenic survival or (/sup 3/H)-thymidine uptake, nor were there any significant differences between the two groups in the induction of DNA strand breaks after gamma irradiation under repairing (37 degrees C) or nonrepairing (0 degrees C) conditions. The results suggest that selenium-dependent glutathione peroxidase does not contribute significantly to the radiation resistance of cultured mammalian cells.
- Research Organization:
- Swedish Defense Research Establishment, Umea
- OSTI ID:
- 6323702
- Journal Information:
- Radiat. Res.; (United States), Vol. 117:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
RADIOSENSITIVITY
CELL KILLING
RADIOINDUCTION
PEROXIDASES
ENZYME ACTIVITY
SELENIUM
BIOLOGICAL EFFECTS
STRAND BREAKS
CATALASE
DNA REPAIR
GAMMA RADIATION
GENETIC RADIATION EFFECTS
GLUTATHIONE
MICE
SUPEROXIDE DISMUTASE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYMES
GENETIC EFFECTS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RECOVERY
REPAIR
RODENTS
SEMIMETALS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture