Diurnal rhythm of melatonin binding in the rat suprachiasmatic nucleus

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-124-3-1585· OSTI ID:6280066

Laitinen, J T; Castren, E; Vakkuri, O; Saavedra, J M

We used quantitative in vitro autoradiography to localize and characterize 2-/sup 125/I-melatonin binding sites in the rat suprachiasmatic nuclei in relation to pineal melatonin production. In a light:dark cycle of 12:12 h, binding density exhibited significant diurnal variation with a peak at the dark-light transition and a trough 12 hours later. Saturation studies suggested that the decreased binding at light-dark transition might be due to a shift of the putative melatonin receptor to a low affinity state.

• Research Organization: National Institute of Mental Health, Bethesda, MD (USA)
• OSTI ID: 6280066
• Journal Information: Endocrinology; (United States), Vol. 124:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MELATONIN
BIOCHEMICAL REACTION KINETICS
RECEPTORS
BIOLOGICAL LOCALIZATION
AUTORADIOGRAPHY
CHEMICAL COMPOSITION
DAILY VARIATIONS
IODINE 125
PINEAL GLAND
RATS
AMINES
ANIMALS
AROMATICS
AZAARENES
AZOLES
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HETEROCYCLIC COMPOUNDS
INDOLES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
RADIOISOTOPES
REACTION KINETICS
RODENTS
TRYPTAMINES
VARIATIONS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques