The effects of hydrostatic pressure on matrix synthesis in articular cartilage

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Journal of Orthopaedic Research (New York); (USA)

DOI:https://doi.org/10.1002/jor.1100090102· OSTI ID:6275700

Hall, A C; Urban, J P; Gehl, K A ^[1]

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1. University Laboratory of Physiology, Oxford (England)

The direct effects of hydrostatic pressure on matrix synthesis in articular cartilage can be studied independently of the other factors that change during loading. We have found that the influence of hydrostatic pressure on incorporation rates of {sup 35}SO{sub 4} and ({sup 3}H)proline into adult bovine articular cartilage slices in vitro depends on the pressure level and on the time at pressure. Pressures in the physiological range (5-15 MPa) applied for 20 s or for 5 min could stimulate tracer incorporation (30-130%) during the following 2 h, but higher pressures (20-50 MPa) had no effect on incorporation rates. The degree of stimulation in cartilage obtained from different animals was found to vary; in some animals none was seen. Stimulation also varied with position along the joint. Physiological pressures (5-10 MPa) applied continuously for the 2-h incubation period also stimulated incorporation rates, but pressures greater than 20 MPa always produced a decrease that was related to the applied pressure and that was reversible. These results suggests that the hydrostatic pressure that occurs during loading is a signal that can stimulate matrix synthesis rates in articular cartilage.

• OSTI ID: 6275700
• Journal Information: Journal of Orthopaedic Research (New York); (USA), Vol. 9:1; ISSN 0736-0266
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARTILAGE
PRESSURE EFFECTS
GLYCOPROTEINS
BIOSYNTHESIS
CATTLE
HIGH PRESSURE
HYDROSTATICS
PROLINE
SULFATES
SULFUR 35
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RUMINANTS
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques