Proton suicide: general method for direct selection of sugar transport- and fermentation-defective mutants

Journal Article · Thu Nov 01 00:00:00 EST 1984 · J. Bacteriol.; (United States)

OSTI ID:6265347

Winkelman, J W; Clark, D P

A positive selection procedure was devised for bacterial mutants incapable of producing acid from sugars by fermentation. The method relied on the production of elemental bromine from a mixture of bromide and bromate under acidic conditions. When wild-type Escherichia coli cells were plated on media containing a fermentable sugar and an equimolar mixture of bromide and bromate, most of the cells were killed but a variety of mutants unable to produce acid from the sugar survived. Among these mutants were those defective in (i) sugar uptake, (ii) the glycolytic pathway, and (iii) the excretion. There were also novel mutants with some presumed regulatory defects affecting fermentation.

• Research Organization: Southern Illinois Univ., Carbondale
• DOE Contract Number: AC02-82ER12095
• OSTI ID: 6265347
• Journal Information: J. Bacteriol.; (United States), Vol. 160:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESCHERICHIA COLI
SENSITIVITY
MUTANTS
CYTOLOGICAL TECHNIQUES
BIOLOGICAL PATHWAYS
BROMINE
EXCRETION
FERMENTATION
GLYCOLYSIS
SACCHARIDES
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
CLEARANCE
DECOMPOSITION
ELEMENTS
HALOGENS
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
550700* - Microbiology