Cotton cellulose: enzyme adsorption and enzymic hydrolysis

Journal Article · Fri Jan 01 00:00:00 EST 1982 · J. Appl. Polym. Sci.; (United States)

DOI:https://doi.org/10.1002/app.1982.070270925· OSTI ID:6247254

Beltrame, P L; Carniti, P; Focher, B; Marzetti, A; Cattaneo, M

The adsorption of a crude cellulase complex from Trichoderma viride on variously pretreated cotton cellulose samples was studied in the framework of the Langmuir approach at 2-8 degrees. The saturation amount of adsorbed enzyme was related to the susceptibility of the substrates to hydrolysis. In every case the adsorption process was faster by 2-3 orders of magnitude than the hydrolysis step to give end products. For ZnCl/sub 2/-treated cotton cellulose the Langmuir parameters correlated fairly well with the value of the Michaelis constant, measured for its enzymic hydrolysis, and the adsorptive complex was indistinguishable from the complex of the Michaelis-Menten model for the hydrolysis.

• Research Organization: 1st Chim Fis, Univ Milano, Milan, Italy
• OSTI ID: 6247254
• Journal Information: J. Appl. Polym. Sci.; (United States), Vol. 27:9
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
ADSORPTION
IMMOBILIZED ENZYMES
CELLULOSE
ENZYMATIC HYDROLYSIS
COTTON
TRICHODERMA VIRIDE
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SORPTION
TRICHODERMA
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