Kinetics and modeling of hexavalent chromium reduction in Enterobacter cloacae

Journal Article · Tue Jan 05 00:00:00 EST 1993 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260410117· OSTI ID:6238127

Yamamoto, Koji; Kato, Junichi; Yano, Takuo; Ohtake, Hisao ^[1]

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1. Hiroshima Univ. (Japan)

Kinetics of bacterial reduction of toxic hexavalent chromium (chromate: CrO[sub 4][sup [minus]2]) was investigated using batch and fed-batch cultures of Enterobacter cloacae strain HO1. In fed-batch cultures, the CrO[sub 4][sup [minus]2] feed was controlled on the basis of the rate of pH change. This control strategy has proven to be useful for avoiding toxic CrO[sub 3][sup [minus]2] overload. A simple mathematical model was developed to describe the bacterial process of CrO[sub 4][sup [minus]2] reduction. In this model, two types of bacterial cells were considered: induced, CrO[sub 4][sup [minus]2]-resistant cells and uninduced, sensitive ones. Only resistant cells were assumed to be able to reduce CrO[sub 4][sup [minus]2]. These fundamental ideas were supported by the model predictions which well approximated all experimental data. In a simulation study, the model was also used to optimize fed-batch cultures, instead of lengthy and expensive laboratory experiments.

• OSTI ID: 6238127
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 41:1; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
GROWTH
CHROMIUM
BIODEGRADATION
BATCH CULTURE
MATHEMATICAL MODELS
REDUCTION
CHEMICAL REACTIONS
DECOMPOSITION
ELEMENTS
METALS
MICROORGANISMS
TRANSITION ELEMENTS
560300* - Chemicals Metabolism & Toxicology