An improved method for the detection of mutants at the waxy locus in Hordeum vulgare
Journal Article
·
· Environ. Mutagen.; (United States)
Immature barley spikes from greenhouse and field-grown plants were harvested 2-4 days prior to anthesis and prepared for analysis. The improved procedure described permits routine and accurate scoring of large numbers of pollen grains and detection of low frequency forward or reverse mutations at the waxy locus. Reversion frequencies are presented in table form for azide- and gamma-induced mutants. (JMT)
- Research Organization:
- Washington State Univ., Pullman
- DOE Contract Number:
- AM06-76RL02221
- OSTI ID:
- 6220316
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZIDO COMPOUNDS
BIOLOGICAL EFFECTS
BARLEY
MUTATION FREQUENCY
GAMMA RADIATION
GENETIC RADIATION EFFECTS
GENES
MEASURING METHODS
MUTANTS
POLLEN
BIOLOGICAL RADIATION EFFECTS
CEREALS
ELECTROMAGNETIC RADIATION
GAMETES
GENETIC EFFECTS
GERM CELLS
GRAMINEAE
GRASS
IONIZING RADIATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
560141* - Radiation Effects on Plants- Basic Studies- (-1987)
560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)
AZIDO COMPOUNDS
BIOLOGICAL EFFECTS
BARLEY
MUTATION FREQUENCY
GAMMA RADIATION
GENETIC RADIATION EFFECTS
GENES
MEASURING METHODS
MUTANTS
POLLEN
BIOLOGICAL RADIATION EFFECTS
CEREALS
ELECTROMAGNETIC RADIATION
GAMETES
GENETIC EFFECTS
GERM CELLS
GRAMINEAE
GRASS
IONIZING RADIATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
560141* - Radiation Effects on Plants- Basic Studies- (-1987)
560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)