Adsorption of Clostridium thermocellum cellulases onto pretreated mixed hardwood, avicel, and lignin

Journal Article · Mon Sep 20 00:00:00 EDT 1993 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260420715· OSTI ID:6204399

Bernardez, T D; Lyford, K; Hogsett, D A; Lynd, L R ^[1]

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1. Dartmouth College, Hanover, NH (United States). Thayer School of Engineering

Adsorption of Avicel-hydrolyzing activity was examined with respect to: mixed hardwood flour pretreated with 1% sulfuric acid for 9 s at 220C (PTW220), lignin prepared from PTW220 by either acid or enzymatic hydrolysis, and Avicel. Experiments were conducted at 60C for all materials, and also at 25C for PTW220. Based on transient adsorption results and reaction rates, times were selected at which to characterize adsorption at 60C as follows: PTW220, 1 min; lignin, 30 min; and Avicel, 45 min. Similar results were obtained for adsorption of cellulase activity to PTW220 at 25 and 60C, and for lignin prepared by enzymatic and acid hydrolysis. For all materials, adsorption was described well by a Langmuir equation, although the reversibility of adsorption was not investigated. Langmuir affinity constants (L/g) were: PTW220, 109; lignin, 17.9; Avicel, 4.3; cellulose from PTW220, [ge]187. Langmuir capacity constants were 760 for PTW220 and 42 for Avicel; the cellulase binding capacity of lignin appeared to be very high under the conditions examined, and could not be determined. At low and moderate cellulase loadings at least, the majority of cellulase activity adsorbed to PTVV220 is bound to the cellulosic component. The results indicate that PTW220, and its cellulose component in particular, differ radically from Avicel with respect to adsorption. Avicel-hydrolyzing activity and CMC-hydrolyzing activities were found to bind to Avicel with a constant ratio of essentially one, consistent with adsorption of a multi-activity complex.

• OSTI ID: 6204399
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 42:7; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CELLULASE
ENZYME ACTIVITY
CLOSTRIDIUM THERMOCELLUM
GROWTH
LIGNIN
ENZYMATIC HYDROLYSIS
ADSORPTION
BACTERIA
CARBOHYDRATES
CHEMICAL REACTIONS
CLOSTRIDIUM
DECOMPOSITION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SOLVOLYSIS
SORPTION
090900* - Biomass Fuels- Processing- (1990-)