Regulation of expression of a soybean storage protein subunit gene. Progress report

Technical Report · Mon Jul 16 00:00:00 EDT 1984

OSTI ID:6137142

Thompson, J F; Madison, J T

We have found that soybean cotyledons could be cultured in vitro and that the storage proteins were formed essentially as on a plant. When methionine was added to the medium, the cotyledons grew faster, and the methionine content of the protein fraction was increased by over 20 percent. The high methionine content of the protein fraction was found to be due to a shift in the relative amounts of the two major storage proteins. The later effect was the result of methionine treatment suppressing the expression of one storage protein subunit gene. The goal was to determine the mechanism by which methionine is able to regulate the expression of the ..beta..-subunit gene.

• Research Organization: Agricultural Research Service, Ithaca, NY (USA). Northeastern Region
• DOE Contract Number: AI05-83ER13114
• OSTI ID: 6137142
• Report Number(s): DOE/ER/13114-T1; ON: DE85006985
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
METHIONINE
BIOLOGICAL EFFECTS
GENETIC EFFECTS
BIOSYNTHESIS
GLYCINE HISPIDA
IN VITRO
PROTEINS
AMINO ACIDS
CARBOXYLIC ACIDS
DRUGS
LEGUMINOSAE
LIPOTROPIC FACTORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
SYNTHESIS
550400* - Genetics