Physiological properties of a mutant of Pachysolen tannophilus deficient in NADPH-dependent D-xylose reductase

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:6122041

Schneider, H; James, A P ^[1]; Lee, Hung; Kubicek, C P ^[2]

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1. National Research Council of Canada, Ottawa, Ontario (Canada)
2. Technische Universitaet Wien, Vienna (Austria)

A D-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on D-xylose but normal growth on xylitol and D-glucose. Fractionation of cell extracts indicated that the mutant was deficient in D-cylose reductase activity that used NADPH exclusively as a cofactor, but not in activity that used both NADH and NADPH. Mutant cultures grown on D-xylose as the sole carbon source exhibited some properties that would be desired in improved strains. Growth rate, growth yield, and D-xylose consumption rate of the mutant were less sensitive than those of the wild type to changes in aeration rate. D-Xylose was utilized more efficiently in that less of a by-product, xylitol, was produced. In addition, under low aeration conditions, more ethanol was produced. A disadvantage was a relatively slow rate of D-xylose utilization.

• OSTI ID: 6122041
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 55:11; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ETHANOL
BIOSYNTHESIS
XYLOSE
METABOLISM
YEASTS
PHYSIOLOGY
NADP
OXIDOREDUCTASES
ALCOHOLS
ALDEHYDES
CARBOHYDRATES
COENZYMES
ENZYMES
EUMYCOTA
FUNGI
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PENTOSES
PLANTS
SACCHARIDES
SYNTHESIS
090900* - Biomass Fuels- Processing- (1990-)