Human papillomavirus type 16 DNA-induced malignant transformation of NIH 3T3 cells

Journal Article · Sat Feb 01 00:00:00 EST 1986 · J. Virol.; (United States)

OSTI ID:6101256

Yasumoto, S; Burkhardt, A L; Doniger, J; DiPaolo, J A

A biological function for human papillomavirus 16 (HPV 16) DNA was demonstrated by transformation of NIH 3T3 cells. HPV 16 DNA has been found frequently in genital cancer and has been classified as a papillomavirus on the basis of DNA homology. A recombinant HPV 16 DNA (pSHPV16d), which contains a head-to-tail dimer of the full-length HPV 16 genome, induced morphologic transformation; the transformed cells were tumorigenic in nude mice. Expression of transforming activity was unique because of the long latency period (more than 4 weeks) required for induction of morphologic transformation and because the transfected DNA existed primarily in a multimeric form with some rearrangement. Furthermore, virus-specific RNAs were expressed in the transformants. The transformation of NIH 3T3 cells provides a model for analyzing the functions of HPV 16, which is associated with cervical carcinomas.

• Research Organization: National Cancer Institute, Bethesda, MD (USA)
• OSTI ID: 6101256
• Journal Information: J. Virol.; (United States), Vol. 57:2
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
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PARASITES
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